Contacto
Campos de conocimiento
Bioquímica
Biotecnología
Farmacología
Reconocimiento Molecular y Bioestructura
Líneas de investigación
Biología Molecular y Bioquímica de venenos de animales ponzoñosos.
Aislamiento y caracterización química y funcional de enzimas y de toxinas de venenos de reptiles.
Desarrollo y mejoramiento de antivenenos.
Farmacocinética de antivenenos en un modelos animal grande (borregos).
Publicaciones
Dobson J, Yang DC, Op den Brouw B, Cochran C, Huynh T, Kurrupu S, Sánchez EE, Massey DJ, Baumann K, Jackson TNW, Nouwens A, Josh P, Neri-Castro E, Alagón A, Hodgson WC, Fry BG. (2018) Rattling the border wall: Pathophysiological implications of functional and proteomic venom variation between Mexican and US subspecies of the desert rattlesnake Crotalus scutulatus. Comp Biochem Physiol C Toxicol Pharmacol. 205: 62-69. doi: 10.1016/j.cbpc.2017.10.008. (FI: 4.52).
Calcines-Cruz C, Olvera A, Castro-Acosta RM, Zavala G, Alagón A, Trujillo-Roldán MA, Valdez-Cruz NA. (2018) Recombinant-phospholipase A2 production and architecture of inclusion bodies are affected by pH in Escherichia coli. Int J Biol Macromol. 108: 826-836. doi: 10.1016/j.ijbiomac.2017.10.178. (FI: 8.025).
Borja M, Neri-Castro E, Castañeda-Gaytán G, Strickland JL, Parkinson CL, Castañeda-Gaytán J, Ponce-López R, Lomonte B, Olvera-Rodríguez A, Alagón A, Pérez-Morales R. (2018) Biological and Proteolytic Variation in the Venom of Crotalus scutulatus scutulatus from Mexico. Toxins (Basel) 8;10(1). pii: E35. doi: 10.3390/toxins10010035. (FI:5.075).
Jaime Felipe Guerrero-Garzón, Melisa Bénard-Valle, Rita Restano-Cassulini, Fernando Zamudio, Gerardo Corzo, Alejandro Alagón, Alejandro Olvera-Rodríguez. (2018) Cloning and sequencing of three-finger toxins from the venom glands of four Micrurus species from Mexico and heterologous expression of an alpha-neurotoxin from Micrurus diastema. Biochimie 147: 114-121. doi: 10.1016/j.biochi.2018.01.006. (FI: 4.372).
Edgar Neri-Castro, Bruno Lomonte, Mariel Valdés, Roberto Ponce-López, Melisa Bénard-Valle, Miguel Borja, Jason L. Strickland, Jason M. Jones, Christoph Grünwald, Fernando Zamudio, Alejandro Alagón. (2018) Venom characterization of the three species of Ophryacus and proteomic profiling of O. sphenophrys unveils Sphenotoxin, a novel Crotoxin-like heterodimeric β-neurotoxin. J Proteomics. 2019 Feb 10;192:196-207. doi: 10.1016/j.jprot.2018.09.002. (FI: 3.855).
Miguel Borja, Edgar Neri-Castro, Rebeca Pérez-Morales, Jason L. Strickland, Roberto Ponce-López, Christopher L. Parkinson, Jorge Espinosa-Fematt, Jorge Sáenz-Mata, Esau Flores-Martínez, Alejandro Alagón and Gamaliel Castañeda-Gaytán. (2018) Ontogenetic Change in the Venom of Mexican Black-Tailed Rattlesnakes (Crotalus molossus nigrescens). Toxins (Basel). 2018 Dec 1;10(12). pii: E501. doi: 10.3390/toxins10120501. (FI:5.075).
Roman-Dominguez,L., Neri-Castro,E., Vazquez-Lopez,H., Garcia-Osorio,B., Archundia,I.G., Ortiz-Medina,J.A., Petricevich,V.L., Alagon,A., Benard-Valle,M. (2019). Biochemical and immunochemical characterization of venoms from snakes of the genus Agkistrodon. Toxicon X, 4, 100013. (FI: 3.2)
Neri-Castro, E. Hernandez-Davila, A. Olvera-Rodriguez, A. Cardoso-Torres, H. Benard-Valle, M. Bastiaans, E. Lopez-Gutierrez, O. Alagon, A. (2019). Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico. Toxicon X, 2, 100007. https://doi.org/10.1016/j.toxcx.2019.100007. (FI: 3.2)
Kakumanu,R. Hodgson,W.C. Ravi,R. Alagón,A. Harris,R.J. Brust,A. Alewood,P.F. Kemp-Harper,B.K. Fry,B.G. (2019). Vampire Venom: Vasodilatory Mechanisms of Vampire Bat (Desmodus rotundus) Blood Feeding. Toxins (Basel), Jan 8;11(1). pii: E26. doi: 10.3390/toxins11010026. (FI:5.075).
Paniagua D, Vergara I, Román R, Romero C, Benard-Valle M, Calderón A, Jiménez L, Bernas MJ, Witte MH, Boyer LV, Alagón A. (2019) Antivenom effect on lymphatic absorption and pharmacokinetics of coral snake venom using a large animal model. Clin Toxicol (Phila). 57(8):727-734. doi: 10.1080/15563650.2018.1550199. (FI: 4.467).
de la Rosa G, Olvera F, Archundia IG, Lomonte B, Alagón A, Corzo G. (2019) Horse immunization with short-chain consensus α-neurotoxin generates antibodies against broad spectrum of elapid venomous species. Nat Commun. 10(1):3642. doi: 10.1038/s41467-019-11639-2. (FI: 17.69).
Sanchez,M., Solano,G., Vargas,M., Reta-Mares,F., Neri-Castro,E., Alagon,A., Sanchez,A., Villalta,M., Leon,G., Segura,A. (2020). Toxicological profile of medically relevant Crotalus species from Mexico and their neutralization by a Crotalus basiliscus/Bothrops asper antivenom. Toxicon, 179, 92-100. doi.org/10.1016/j.toxicon.2020.03.006. (FI: 3.035).
Rivas-Mercado E., Neri-Castro E., Benard-Valle M., Rucavado-Romero,A., Olvera-Rodriguez A., Zamudio-Zuniga F., Alagon,A., Garza-Ocanas L. (2020). Disintegrins extracted from totonacan rattlesnake (Crotalus totonacus) venom and their anti-adhesive and anti-migration effects on MDA-MB-231 and HMEC-1 cells. Toxicology in Vitro, 65, 104809. doi.org/10.1016/j.tiv.2020.104809. (FI: 3.685).
Ponce-Lopez,R., Neri-Castro,E., Borja,M., Strickland,J.L., Alagon,A. (2020). Neutralizing potency and immunochemical evaluation of an anti-Crotalus mictlantecuhtli experimental serum. Toxicon, 187, 171-180. doi.org/10.1016/j.toxicon.2020.08.026. (FI: 3.035).
Paniagua, D., Crowns, K., Montonera, M., Wertheimer, A., Alagon, A, Boyer, L. Postmortem histopathology and detection of venom by ELISA following suicide by cobra (Naja kaouthia) envenomation. Forensic Toxicol. 38, 523–528 (2020). doi.org/10.1007/s11419-020-00535-w. (FI: 2.855).
Neri-Castro, E., Sanz, L., Olvera-Rodriguez, A., Benard-Valle, M., Alagon, A., Calvete,J.J. (2020). Venomics and biochemical analysis of the black-tailed horned pitviper, Mixcoatlus melanurus, and characterization of Melanurutoxin, a novel crotoxin homolog. Journal of Proteomics, 225, 103865. doi.org/10.1016/j.jprot.2020.103865. (FI: 3.855).
Neri-Castro,E., Benard-Valle,M., Paniagua,D., Boyer,L.V., Possani,D., Lopez-Casillas,F., Olvera,A., Romero,C., Zamudio,F., Alagon,A. (2020). Neotropical Rattlesnake (Crotalus simus) Venom Pharmacokinetics in Lymph and Blood Using an Ovine Model. Toxins (Basel), 12 (7), 455. doi.org/10.3390/toxins12070455. (FI:5.075).
Grashof,D., Zdenek,C.N., Dobson,J.S., Youngman,N.J., Coimbra,F., Benard-Valle,M., Alagon,A., Fry,B.G. (2020). A Web of Coagulotoxicity: Failure of Antivenom to Neutralize the Destructive (Non-Clotting) Fibrinogenolytic Activity of Loxosceles and Sicarius Spider Venoms. Toxins (Basel), 12 (2). doi.org/10.3390/toxins12020091. (FI:5.075).
Garcia-Osorio,B., Lomonte,B., Benard-Valle,M., Lopez de Leon J., Roman-Dominguez,L., Mejia-Dominguez,N., Lara-Hernandez,F., Alagon,A., Neri-Castro,E. (2020). Ontogenetic changes in the venom of Metlapilcoatlus nummifer, the Mexican jumping viper. Toxicon, 184, 204-214. doi.org/10.1016/j.toxicon.2020.06.023. (FI: 3.035).
Chippaux,J.P., Celis,A., Boyer,L., Alagon,A. (2020). Factors involved in the resilience of incidence and decrease of mortality from scorpion stings in Mexico. Toxicon, 188, 65-75. doi.org/10.1016/j.toxicon.2020.10.011. (FI: 3.035).
Braga, J.R.M., Jorge, A.R.C., Marinho, A.D., de Moraes Silveira, J.A., Nogueira-Junior, F.A., Benard-Valle, M., Alagon, A., de Menezes, R.R.P.P., Martins, A.M.C., Feijao, L.X., Monteiro, H.S.A., Jorge, R.J.B. (2020). Renal effects of venoms of Mexican coral snakes Micrurus browni and Micrurus laticollaris. Toxicon, 181, 45-52. doi.org/10.1016/j.toxicon.2020.04.095. (FI: 3.035).
Benard-Valle, M., Neri-Castro, E.E., Yanez-Mendoza, M.F., Lomonte, B., Olvera, A., Zamudio, F., Restano-Cassulini, R., Possani, L.D., Jimenez-Ferrer, E., Alagon, A. (2020). Functional, proteomic and transcriptomic characterization of the venom from Micrurus browni browni: Identification of the first lethal multimeric neurotoxin in coral snake venom. Journal of Proteomics, 225, 103863. doi.org/10.1016/j.jprot.2020.103863. (FI: 3.855).
Seneci, L., Zdenek, C.N., Bourke, L.A., Cochran, C., Sanchez, E.E., Neri-Castro, E., Benard-Valle, M., Alagon, A., Frank, N., Fry, B.G. (2021). A symphony of destruction: Dynamic differential fibrinogenolytic toxicity by rattlesnake (Crotalus and Sistrurus) venoms. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 245, 109034. doi.org/10.1016/j.cbpc.2021.109034. (FI: 3.228).
Seneci, L., Zdenek, C.N., Chowdhury, A., Rodrigues, C.F.B., Neri-Castro, E., Benard-Valle, M., Alagon, A., Fry, B.G. (2021). A Clot Twist: Extreme Variation in Coagulotoxicity Mechanisms in Mexican Neotropical Rattlesnake Venoms. Frontiers in Immunology, 12, 612846. doi.org/10.3389/fimmu.2021.612846. (FI: 6.429).
Rodrigues, C.F.B., Zdenek, C.N., Serino-Silva, C., de Morais-Zani, K., Grego, K.F., Benard-Valle, M., Neri-Castro, E., Alagon, A., Tanaka-Azevedo, A.M., Fry, B.G. (2021). Boa g PLI from Boa constrictor Blood is a Broad-Spectrum Inhibitor of Venom PLA2 Pathophysiological Actions. Journal of Chemical Ecology, 47, 907–914 (2021). doi.org/10.1007/s10886-021-01289-4. (FI: 2.239).
Ponce-Lopez, R., Neri-Castro, E., Olvera-Rodriguez, F., Sanchez, E.E., Alagon, A., Olvera-Rodriguez, A. (2021). Neutralization of crotamine by polyclonal antibodies generated against two whole rattlesnake venoms and a novel recombinant fusion protein. Toxicon, 197, 70-78. doi.org/10.1016/j.toxicon.2021.04.005. (FI: 3.035).
Franco-Servin, C., Neri-Castro, E., Benard-Valle, M., Alagon, A., Rosales-Garcia, R.A., Guerrero-Alba, R., Poblano-Sanchez, J.E., Silva-Briano, M., Guerrero-Barrera, A.L., Sigala-Rodriguez, J.J. (2021). Biological and Biochemical Characterization of Coronado Island Rattlesnake (Crotalus helleri caliginis) Venom and Antivenom Neutralization. Toxins (Basel), 13 (8), 582. doi.org/10.3390/toxins13080582. (FI:5.075).
de Roodt, A.R., Lanari, L.C., Ramirez, J.E., Gomez, C., Barragan, J., Litwin, S., van Grootheest, J.H., Desio, M., Dokmetjian, J.C., Dolab, J.A., Damin, C.F., Alagon, A. (2021). Cross-reactivity of some Micrurus venoms against experimental and therapeutic anti-micrurus antivenoms. Toxicon, 200, 153-164. doi.org/10.1016/j.toxicon.2021.07.011. (FI: 3.035).
Dashevsky, D., Benard-Valle, M., Neri-Castro, E., Youngman, N.J., Zdenek, C.N., Alagon, A., Portes-Junior, J.A., Frank, N., Fry, B.G. (2021). Anticoagulant Micrurus venoms: targets and neutralization. Toxicology Letters, 337, 91-97. doi.org/10.1016/j.toxlet.2020.11.010. (4.372).
Colis-Torres, A., Neri-Castro, E., Strickland, J.L., Olvera-Rodriguez, A., Borja, M., Calvete, J., Jones, J., Parkinson, C.L., Banuelos, J., Lopez de Leon, J., Alagon, A. (2021). Intraspecific venom variation of Mexican West Coast Rattlesnakes (Crotalus basiliscus) and its implications for antivenom production. Biochimie, 192: 111-124. doi.org/10.1016/j.biochi.2021.10.006. (FI: 4.372).
Bourke, L.A., Zdenek, C.N., Neri-Castro, E., Benard-Valle, M., Alagon, A., Gutierrez, J.M., Sanchez, E. F., Aldridge, M., Fry, B.G. (2021). Pan-American Lancehead Pit-Vipers: Coagulotoxic Venom Effects and Antivenom Neutralisation of Bothrops asper and B. atrox Geographical Variants. Toxins (Basel), 13 (2), 78. doi.org/10.3390/toxins13020078. (FI: 5.075)
Benard-Valle, M., Neri-Castro, E., Elizalde-Morales, N., Olvera-Rodriguez, A., Strickland, J., Acosta, G., Alagon, A. (2021). Protein composition and biochemical characterization of venom from Sonoran Coral Snakes (Micruroides euryxanthus). Biochimie, 182, 206-216. doi.org/10.1016/j.biochi.2021.01.003. (FI: 4.372).
Archundia, I.G., de la Rosa, G., Olvera, F., Calderon, A., Benard-Valle, M., Alagon, A., Corzo, G. (2021). Assessment of neutralization of Micrurus venoms with a blend of anti-Micrurus tener and anti-ScNtx antibodies. Vaccine, 39 (6), 1000-1006. doi.org/10.1016/j.vaccine.2020.12.052. (FI:4169).
Youngman, N.J., Carlsson, D.J., Jones, L., Neri-Castro, E., Alagon, A., Fry, B.G. (2022). Cloud serpent coagulotoxicity: The biochemical mechanisms underpinning the anticoagulant actions of Mixcoatlus and Ophryacus venoms. Toxicon, 211, 44-49. (FI: 3.035).
Neri-Castro, E., Strickland, J.L., Carbajal-Marquez, R.A., Zuniga, J., Ponce-Lopez, R., Olvera, F., Alagon, A. (2022). Characterization of the venom and external morphology of a natural hybrid between Crotalus atrox and Crotalus mictlantecuhtli. Toxicon, 207, 43-47. (FI: 3.035).
Colis-Torres, A., Neri-Castro, E., Strickland, J.L., Olvera-Rodriguez, A., Borja, M., Calvete, J., Jones, J., Parkinson, C.L., Banuelos, J., Lopez de Leon, J., Alagon, A. (2022). Intraspecific venom variation of Mexican West Coast Rattlesnakes (Crotalus basiliscus) and its implications for antivenom production. Biochimie, 192, 114-124. doi: 10.1016/j.biochi.2021.10.006. (FI: 4.372).
Olvera Rodríguez, A., Amaro Ruiz, MG., Bénard-Valle, M., Neri-Castro, E., Olvera
Rodríguez, F., Alagón, A. (2022) Neutralization of black widow spider (Latrodectus mactans) venom with rabbit polyclonal serum hyperimmunized with recombinant alpha-latrotoxin fragments. Biochimie. 201:55-62. doi:10.1016/j.biochi.2022.06.012. (FI: 4.372).
Neri-Castro, E., Zarzosa, V., Colis-Torres, A., Fry, BG., Olvera-Rodríguez, A., Jones, J., Reyes-Velasco, J., Zamudio, F., Borja, M., Alagón, A., Lomonte, B. Proteomic and toxicological characterization of the venoms of the most enigmatic group of rattlesnakes: The long-tailed rattlesnakes. (2022) Biochimie, 202, 226-236. doi: 10.1016/j.biochi.2022.08.015. (FI: 4.372).
Siegrist,A.A., Boyer,L., Balchan,N.R., Vazquez,H., Alagon,A., de Roodt,A., Craig,I.S., Poulin,I.S., Timmerman,A., Trocola,D. (2022). Snake and Scorpion Antivenom in Birds of Prey. Journal of Zoo and Wildlife Medicine, 53(4), 870-806, doi: 10.1638/2021-0124. (FI: 0.806).
