Contacto
Campos de conocimiento
Biofísica
Bioinformática
Biología del Desarrollo
Genética y Fisiología Celular
Reconocimiento Molecular y Bioestructura
Líneas de investigación
Estudio de la regulación de la movilidad del espermatozoide enfocando 4 proteínas: CatSper, sNHE (intercambiador sodio/protón del espermatozoide), sAC (adenilil ciclase soluble) y canales de potacio especifico del espermatozoide (TetraKCNG y Slo3).
Interacción molecular usando proteínas fluorescentes particularmente usando la técnica de FRET(Transferencia de Energia por Resonancia)
Publicaciones
*47. César Arcos-Hernández, Esteban Suárez-Delgado, León D. Islas, Francisco Romero, Ignacio López-González, Hui-wang Ai, Takuya Nishigaki. 2023. How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger. Protein Expression and Purification 201, 106172
*46 Hernández-Garduño S, Chávez JC, Matamoros-Volante A, Sánchez-Guevara Y, Torres P, Treviño CL and Nishigaki T. 2022. Hyperpolarization induces cytosolic alkalization of mouse sperm flagellum probably through sperm Na+/H+ exchanger. Reproduction 164, 125-134
45. De La Rosa JAM, García-Castañeda M, Nishigaki T, Gómora JC, Mancilla-Percino T, Ávila G. 2020. Interaction of MDIMP with the Voltage-Gated Calcium Channels. Mol Pharmacol. 98:211-221. doi: 10.1124/mol.120.119982. PMID: 32587097
44. Darszon A, Nishigaki T, López-González I, Visconti PE, Treviño CL. 2020. Differences and Similarities: The Richness of Comparative Sperm Physiology. Physiology (Bethesda). 2020 35:196-208. doi: 10.1152/physiol.00033.2019. PMID: 32293232
43. Garza-López E, Aldana A, Darszon A, Nishigaki T, López-González I. 2020. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II. Cell Calcium. 89, 102214. doi: 10.1016/j.ceca.2020.102214. PMID: 32428730
42 Priego-Espinosa DA, Darszon A, Guerrero A, González-Cota AL, Nishigaki T, Martínez-Mekler G, Carneiro J. 2020. Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm. PLoS Comput Biol. 2;16(3):e1007605. doi: 10.1371/journal.pcbi.1007605. eCollection 2020 Mar. PMID: 32119665
*41 Chávez JC, Darszon A, Treviño CL and Nishigaki T. 2020. Quantitative intracellular pH determinations in single live mammalian spermatozoa using the ratiometric dye SNARF-5F. Front. Cell Dev. Biol. 17, 366. doi: 10.3389/fcell.2019.00366. PMID: 32010689
*40 Romero F, Nishigaki T. 2019 Comparative genomic analysis suggests that the sperm-specific sodium/proton exchanger and soluble adenylyl cyclase are key regulators of CatSper among the Metazoa. Zoological Lett. 5, 25. (doi: 10.1186/s40851-019-0141-3).
*39 Kinoshita-Terauchi N, Shiba K, Terauchi M, Romero F, Ramírez-Gómez HV, Yoshida M, Motomura T, Kawai H, Nishigaki T. 2019 High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga. Zygote 27, 225-231. (doi: 10.1017/S0967199419000224).
*38 Santana-Calvo C, Romero F, López-González I and Nishigaki T. 2018. Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor. BioTechniques 65, 211-218 (doi: 10.2144/btn-2018-0041).
37 Chávez JC, De la Vega-Beltrán JL, José O, Torres P, Nishigaki T, Treviño CL and Darszon A. 2018. Acrosomal alkalization triggers Ca2+ release and acrosome reaction in mammalian spermatozoa. AR. J. Cell Physiol. 233, 4735-4747 (doi: 10.1002/jcp.26262).
